LIFE SCIENCE

MULTI-PHOTON - Z-stack of 3D nano-printed scaffold imitating native lung tissue (20x, 0.75 NA, FOV 600 µm; stepsize 3 µm) - overlay of 2P-460 nm (Hoechst, blue), 2P-542nm (Rose Bengal, green) & 2P-595 nm (Phalloidin-ATTO-490LS, red). Sample: HM Munich

MULTI-PHOTON - Swine olfactory epithelium (20x, 0.75 NA, FOV: 16.1 x 3.7 mm); cell nuclei stained with DAPI and NOGO-A stained with RhodamineRedX. Overlay of 2P-460 nm (green), SHG-520nm (green) and 2P-595 nm (red). Sample: HBC Biberach

MULTI-PHOTON - Living human epithelial cells (60x, 1.4 NA, FOV 90 x 150 µm) - 2P-460 nm (Hoechst/red) & 2P-595 nm (Spirochrome SPY555-tubulin/green). Sample: VIVIT, Dornbirn

MULTI-PHOTON - Time lapse of human epithelial cells (60x, 1.4 NA, FOV 665 µm) - Overlay of 2P-460 nm (Hoechst, blue) & 2P-595 nm (SPY555-tubulin, green). DLD1 cells grew in ibidi 8-Well Slide for 1 day. Sample: VIVIT

MULTI-PHOTON - cellular spheroids (60x, 1.4 NA, FOV 80 µm) - overlay of 2P-460 nm (Hoechst, grey) & 2P-595 nm (Spirochrome SPY555-tubulin, orange). DLD1 cells grew in an ibidi 8-Well Slide for 1 day; Sample: VIVIT, Dornbirn

MULTI-PHOTON - dividing cell embedded in gel with fluorescent beads (60x, 1.4 NA, z-stack: stepsize 0.2 µm) - 542 nm (green) 595 nm (yellow) & 700 nm (red). Staining: cell nuclei w/ mCherry, actin w/ Atto490LS & green-fluorescent beads. Sample: LMU München

WIDEFIELD - DAPI-stained mouse head (sagittal plane; 4x, 0.2 NA) - overlay of 395/432 nm (blue), 475/515 nm (green), 637/682 nm (red). Sample: University of Applied Sciences Biberach

MULTI-PHOTON - ZEBRAFISH Contact us for more details; Sample: Univeristy of Zurich

MULTI-PHOTON - ZEBRAFISH Contact us for more details; Sample: Univeristy of Zurich

LIGHT SHEET - Maximum intensity projection of unlabeled honey bee (0.63x, 0.15 NA, FOV 21 x 21 mm) - excitation at 488 nm (blue); clearing: BABB over 2 weeks.

LIGHT SHEET - Z-stack of unlabeled honey bee (0.63x, 0.15 NA, FOV 21 x 21 mm) - excitation at 488 nm (blue); clearing: BABB over 2 weeks.

LIGHT SHEET - Maximum intensity projection of unlabeled, unknown mushroom (1x, 0.15 NA, FOV 10 x 12 mm) - autofluorescence 488 nm (blue); clearing: BABB.

LIGHT SHEET - Animation of unlabeled, unknown mushroom (1x, 0.15 NA, FOV 10 x 12 mm) - autofluorescence 488 nm (blue); z-stack: step size 5 µm over 7.7 mm in depth; clearing: BABB.

LIGHT SHEET - Z-stack of unstained chicken embryo E8 (age: 8 d; 1x, 0.15 NA, FOV 10x10 mm) - autofluorescence 488 nm (blue); z-stack: step size 5 µm over 9.8 mm in depth; BABB cleared. Sample kindly provided by M. Schaettin (University of Zürich).

MULTI-PHOTON - Z-stack of healthy mouse skin section stained with SiR-DNA (16x, 0.8 NA, FOV 300 µm) - 2P-700 nm (red), SHG-515 nm (green), z-stack: 300 µm depth with 1 µm step size.

MULTI-PHOTON - Animation of healthy mouse skin section stained with SiR-DNA (16x, 0.8 NA, FOV 300 µm) - 2P-700 nm (red), SHG-515 nm (green).

WIDEFIELD - DAPI-stained mouse head (sagittal plane; 4x, 0.2 NA) - overlay of 395/432 nm (blue), 475/515 nm (green), 637/682 nm (red). Sample: University of Applied Sciences Biberach

MULTI-PHOTON - DAPI-stained healthy mouse knee cryosection (25x, NA 1.0, 700 μm) - overlay of 2P-460 nm (blue), SHG-515 (green) and 2P-542 nm (red). Sample kindly provided by B. Hartmann, CANTER lab & Hochschule München

MULTI-PHOTON - Z-stack image of an unstained healthy mouse knee cryosection, 16x, 0.8 NA, 600 µm, SHG-520 nm (blue), 2P-542 nm (green), 2P-700 nm (red).

MULTI-PHOTON - Unstained healthy mouse knee cryosection,16x, NA 0.8, 900 µm, SHG- 520 nm and 2P-595 nm

MULTI-PHOTON - Unstained healthy mouse knee cryosection,16x, NA 0.8, 300 µm, SHG- 520 nm and 2P-595 nm

MULTI-PHOTON - Z-stack of unstained mouse knee cryosection (16x, NA 0.8, FOV 900 µm) - SHG-520 nm

MULTI-PHOTON - Unstained healthy mouse tail cryosection - 16x, NA 0.8, FOV 600 µm - SHG-520 nm (green) and 2P-595 (red)

MULTI-PHOTON - Stained healthy neural tissue cryosection, 16x, NA 0.8

MULTI-PHOTON - Proof of concept for artificial artery (SHG-515 nm, 900 µm, z-stack: 500 µm depth with 10 µm step size). Image kindly provided by D. Bastl (superv. by Dr. S. Sudhop), CANTER lab & Hochschule München.

WIDEFIELD - Stitched single-photon image of an unstained healthy mouse spleen cryosection (16x, 0.8 NA, 10.3 x 6.7 mm) - overlay of 432 nm (blue), 515 nm (green) and 595 nm (red).

MULTI-PHOTON - Z-stack of 3D nano-printed scaffold imitating native lung tissue (20x, 0.75 NA, FOV 600 µm; stepsize 3 µm) - overlay of 2P-460 nm (Hoechst, blue), 2P-542nm (Rose Bengal, green) & 2P-595 nm (Phalloidin-ATTO-490LS, red). Sample: HM Munich

MULTI-PHOTON - Swine olfactory epithelium (20x, 0.75 NA, FOV: 16.1 x 3.7 mm); cell nuclei stained with DAPI and NOGO-A stained with RhodamineRedX. Overlay of 2P-460 nm (green), SHG-520nm (green) and 2P-595 nm (red). Sample: HBC Biberach

MULTI-PHOTON - Healthy FA-fixed swine epithel cryosection (60x, 1.4 NA, FOV 60 x 40 µm, pixel size: 0.078 µm, z-stack: stepsize 0.3 µm) stained with Hoechst 460 nm (blue). Sample: HBC Biberach.

MULTI-PHOTON - Z-stack of healthy FA-fixed swine epithel cryosection (60x, 1.4 NA, FOV 60 x 40 µm, pixel size: 0.078 µm, z-stack: stepsize 0.3 µm) stained with Hoechst 460 nm (blue). Sample: HBC Biberach.

WIDEFIELD - Stitched image of unstained healthy human lung cryosection (16x, 0.8 NA, 11.3 x 7 mm) - overlay of 432 nm (blue), 515 nm (green) and 681 nm (red).

WIDEFIELD - Stitched image of a healthy mouse lung, 16 x 0.8 NA

MULTI-PHOTON - Cross section of healthy FA-fixed mouse colon stained with Hoechst imaged from the whole organ (16x, 0.8 NA, FOV 2.7 x 3.8 mm, pixel size: 0.32 µm) - overlay of Hoechst 460 nm (blue), SHG-515 nm (green), autofluorescence 595 nm (red).

MULTI-PHOTON - Z-stack of healthy unstained FA-fixed mouse colon (16x, 0.8 NA, FOV 570 µm) - SHG-515 nm (blue), 2P-542 nm (green) and 2P-700 nm (red); z-stack: step size 5 µm over 230 µm in depth.

MULTI-PHOTON - Unstained human prostate (40x, NA 0.95, FOV 143 µm) - SHG-515 nm (magenta), 2P-542 (blue), 2P-625 (green) and 2P-700 (red)

MULTI-PHOTON - Unstained healthy fresh swine kidney (16x, 0.8 NA, FOV 300 µm) - SHG-515 nm (blue), 2P-542 nm (green) and 2P-700 nm (red).

MULTI-PHOTON - Unstained healthy fresh swine kidney (16x, 0.8 NA, FOV 300 µm) - SHG-515 nm (blue), 2P-542 nm (green) and 2P-700 nm (red).

MULTI-PHOTON - Unstained healthy fresh swine kidney (16x, 0.8 NA, FOV 300 µm) - SHG-515 nm (blue), 2P-542 nm (green) and 2P-700 nm (red).

MULTI-PHOTON - Z-stack image of a sunflower pollen. You can clearly see all the details of the pollen.

MULTI-PHOTON - Living human epithelial cells (60x, 1.4 NA, FOV 90 x 150 µm) - 2P-460 nm (Hoechst/red) & 2P-595 nm (Spirochrome SPY555-tubulin/green). Sample: VIVIT, Dornbirn

MULTI-PHOTON - Time lapse of human epithelial cells (60x, 1.4 NA, FOV 665 µm) - Overlay of 2P-460 nm (Hoechst, blue) & 2P-595 nm (SPY555-tubulin, green). DLD1 cells grew in ibidi 8-Well Slide for 1 day. Sample: VIVIT

MULTI-PHOTON - 3d projection of human epithelial cells (60x, 1.4 NA, FOV 665 µm) - Overlay of 2P-460 nm (Hoechst, blue) & 2P-595 nm (SPY555-tubulin, green). DLD1 cells grew in ibidi 8-Well Slide for 1 day. Sample: VIVIT

MULTI-PHOTON - cellular spheroids (60x, 1.4 NA, FOV 80 µm) - overlay of 2P-460 nm (Hoechst, grey) & 2P-595 nm (Spirochrome SPY555-tubulin, orange). DLD1 cells grew in an ibidi 8-Well Slide for 1 day; Sample: VIVIT, Dornbirn

MULTI-PHOTON - dividing cell embedded in gel with fluorescent beads (60x, 1.4 NA, z-stack: stepsize 0.2 µm) - 542 nm (green) 595 nm (yellow) & 700 nm (red). Staining: cell nuclei w/ mCherry, actin w/ Atto490LS & green-fluorescent beads. Sample: LMU München

MULTI-PHOTON - ICC-stained prim. cells from porcine olfactory epithelium (60x, 1.4NA, FOV 80µm, pxsize: 0.078µm). Staining: DAPI - cell nuclei, AF488 - acetyl ß-tubulin. 460nm (blue) & 542nm (red); z-stack: step sz 0.1µm, depth 2.6µm; S: HBC Biberach

MULTI-PHOTON - ICC-stained primary cells from porcine olfactory epithelium (60x, 1.4NA, FOV 80µm, px size: 0.078µm). Staining: DAPI - cell nuclei, AF488 - acetylated ß-tubulin. 460nm (blue) & 542nm (red); z-stack: stepsize 0.1µm; Sample: HBC Biberach

MULTI-PHOTON - ICC-stained primary cells from porcine olfactory epithelium (60x, 1.4 NA, FOV 160 µm, px size: 0.078 µm); Staining: DAPI - cell nuclei (460nm, blue); AF488 - ZO-1 (542nm, green); AF647 - E-Cadherin (700nm, red). Sample: HBC Biberach

MULTI-PHOTON - Stained mouse fibroblasts (60x, 1.4 NA, FOV 160 µm) - 460 nm (blue), 542 nm (green) & 595 nm (red). Staining: DAPI - cell nuclei; Alexa Fluor 488 - microtubuli; Lifeact-RFP - actin filaments. Sample: University Konstanz.

WIDEFIELD - Stained mouse fibroblast (100x, 1.4 NA, FOV 135 µm) - 432 nm (blue), 515 nm (green) & 595 nm (red). Staining: DAPI - cell nucleus; Alexa Fluor 488 - microtubuli & Lifeact-RFP - actin filaments. Sample: University Konstanz.

WIDEFIELD - Stained mouse fibroblasts (60x, 0.8 NA, FOV 130 µm) – 432 nm (blue) and 681 nm (red). DAPI stained cell nucleus; HiLyte647 stained microtubuli

WIDEFIELD - Stained mouse fibroblasts (60x, 0.8 NA, FOV 90 µm) – 432 nm (blue) and 681 nm (red). DAPI stained cell nucleus; HiLyte647 stained microtubuli